2017 7: 46067.ĭetermination of TrkB binding affinity of the isolated compounds using CMAC columns (cont’d)ġ7. E.E. Discovery of new muscarinic acetylcholine receptor antagonists from Scopolia tangutica. 2015 Feb 14(2):111-29ĭetermination of TrkB binding affinity of the isolated compounds using CMAC columnsġ6. Du et al. The re-emergence of natural products for drug discovery in the genomics era. Isolation of secondary metabolites identified as specifically binding to targets immobilized on CMAC columns (cont’d)ġ5. Harvey et al. Neurohormetic phytochemicals: An evolutionary-bioenergetic perspective. Neurochem Int. Isolation of secondary metabolites identified as specifically binding to targets immobilized on CMAC columns (cont’d)ġ4. V. Plant alkaloids as drug leads for Alzheimer's disease. Isolation of secondary metabolites identified as specifically binding to targets immobilized on CMAC columnsġ3. Y.P. Magnetic beads-based neuraminidase enzyme microreactor as a drug discovery tool for screening inhibitors from compound libraries and fishing ligands from natural products. Fraction testing using CMAC columns (cont’d)ġ2. Y.M. Moaddel, Comparison of analytical techniques for the identification of bioactive compounds from natural products. Raguso RA, Why do plants produce so many terpenoid compounds? New Phytol. Screening extracts for certain classes of secondary metabolites: b) terpenoidsġ0. E. Screening extracts for certain classes of secondary metabolites: b) coumarins, flavonoids and phenolic acidsĩ. M. Plant extract fingerprinting and fractionation (cont’d) Are tropane alkaloids present in organic foods? Detection of scopolamine and atropine in organic buckwheat (Fagopyron esculentum L.) products by UHPLC-MS/MS. Screening extracts for certain classes of secondary metabolites: a) alkaloidsĨ. M. Plant extract fingerprinting and fractionation. Frontal affinity chromatography-mass spectrometry useful for characterization of new ligands for GPR17 receptor. 2016 Jan 29 1431: 138–144.ĬMAC column characterization using frontal affinity chromatography determination of KD value of a known ligand determination of the number of binding sites ħ. E. Development and characterization of the α3β4α5 nicotinic receptor cellular membrane affinity chromatography column and its application for on line screening of plant extracts. Preparation of cellular membrane affinity chromatography (CMAC) columns using cultured cellsĦ. L. Visit to the UA Herbarium to collect/obtain plant specimensĥ. Methods optimization in Accelerated Solvent Extraction, Technical Note 208, Thermo Scientific. Safety, SOPs (*1-2) and Ben Shen paper (*3). Hospice is the most ideal program for this purpose.2. SOP for BSL-1 and SOP for liquid-liquid extraction using organic solventsĬulturing cell line practices (checking cells, changing medium, splitting, passage number, etc).ģ. Ben Shen, A New Golden Age of Natural Products Drug Discovery, Cell. Thus, it is important for the healthcare team to adopt sensitive and systematic approach to patients including documentation and communication of end-of-life issues. This is a time when levels of anxiety, stress, and emotion can be high to patients, families, and other caregivers. Control of symptoms and family support are extremely important, and the actual nature of the primary illness takes less importance. Terminal patients also experience increasing weakness and immobility, loss of interest in food and drink, difficulty in swallowing, and drowsiness during the last hours of life. However, inevitable problems accompanied by, pain, dyspnea, moist breathing, nausea and vomiting, restlessness jerking and twitching were commonly noted in the last phase of life. Caring for a patient dying is a hardship not only to the health care professionals, but also to the direct family members.

Sylaps scopolamine